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Immunology and Infection

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JoVE Journal Immunology and Infection
Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
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Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease

Article DOI: 10.3791/60083-v • 08:05 min • March 17th, 2020
March 17th, 2020 •
*1, *2, 1, 1, 1, 1, 1, 3, 4,5
1Cancer Division, Burnett School of Biomedical Sciences, University of Central Florida, 2Department of Biochemistry, Hanoi University of Pharmacy, 3Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences, Houston Methodist Research Institute, 4Department of Epidemiology, University of Pittsburgh Graduate School of Public Health, 5Division of Cancer Control and Population Sciences, University of Pittsburgh Medical Center, Hillman Cancer Center
* These authors contributed equally

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Summary
Automatic Translation

Graft-versus-host disease is a major complication after allogeneic bone marrow transplantation. Dendritic cells play a critical role in the pathogenesis of graft-versus-host disease. The current article describes a novel bone marrow transplantation platform to investigate the role of dendritic cells in the development of graft-versus-host disease and the graft-versus-leukemia effect.

Tags

Bone Marrow Transplantation Platform Dendritic Cells Graft-versus-host Disease Ex Vivo Cell Regeneration Immune Cell Population Macrophage Neutrophils Lab Manager Undergraduate T-cell-depleted Bone Marrow Preparation Femur Tibia 1% RPMI Medium Syringe Needle Bone Marrow Collection Cell Strainer Single-cell Suspension Centrifugation THI1 Antibody
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