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Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy

Article DOI: 10.3791/60270-v • 10:26 min • January 30th, 2020
January 30th, 2020 •
1, 1
1Department of Dentistry and Oral Health, Aarhus University

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Summary
Automatic Translation

The protocol describes the cultivation of cross-kingdom biofilms consisting of Candida albicans and Streptococcus mutans and presents a confocal microscopy-based method for the monitoring of extracellular pH inside these biofilms.

Tags

Monitoring Extracellular PH Cross-kingdom Biofilms Confocal Microscopy Streptococcus Mutans Candida Albicans PH Ratiometry Image Acquisition Parameters Horizontal PH Gradients Vertical PH Gradients Fungal Biofilms Bacterial Biofilms Microbial Metabolism Biofilm Growth Anette Aakjaer Thomsen Technician Laboratory Blood Agar Plates Aerobic Conditions Test Tubes Brain Heart Infusion Centrifuge Supernatant Resuspend Cells Physiological Saline OD 550
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