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A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling
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Medizin
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JoVE Journal Medizin
A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling

A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling

DOI:
10.3791/60344-v

•

06:36 min

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November 28, 2019

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, , ,
1Department of Physiology and Cell Biology,University of Nevada Reno School of Medicine, 2Charles River Laboratories Inc.

Kapitel

  • 00:05Titel
  • 00:40Detrusor-Free Bladder Preparation Dissection
  • 02:41Denuded Bladder Preparation Filling and Mediator Detection
  • 03:40Results: Representative Denuded Murine Bladder Preparation Assessment
  • 05:44Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The detrusor-free bladder model enables direct access to the suburothelium to study local mechanisms for regulation of biologically active mediator availability in suburothelium/lamina propria during storage and voiding of urine. The preparation closely resembles filling of an intact bladder and allows pressure-volume studies to be performed without systemic influences.

Tags

Decentralized Bladder ModelDetrusor Muscle RemovalSuburotheliumBladder FillingUrothelial MediatorsSignaling MechanismsBladder ExcitabilityMurine Bladder SpecimenDissectionOxygenated KBSDetrusor Muscle LayerBladder MucosaCatheter InsertionOrgan Dish

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