Journal
/
Biochemie
/
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

DOI:
10.3791/60369-v

•

08:09 min

•

October 15, 2019

•

,
1Department of Biological Sciences,Smith College, 2Center for Microscopy and Imaging,Smith College

Kapitel

  • 00:04Titel
  • 00:46Galactose-Induced Motor Protein Growth, Expression, and Harvesting
  • 01:39Cell Lysis and Soluble Protein Extraction
  • 02:36IgG Affinity Purification
  • 03:17DNA Oligonucleotide Labeling and TEV Cleavage
  • 04:50Chassis Purification
  • 05:56Results: Representative Motor Ensemble Production
  • 07:26Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.

Tags

DNA OrigamiCytoskeletal Motor ProteinsDyneinKinesinMyosinCargo TransportSingle Molecule ObservationYeast ExpressionProtein PurificationIgG Affinity Purification

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Read Article