Lokalisation und Quantifizierung von Begomoviren in Whitefly-Geweben durch Immunfluoreszenz und quantitative PCR
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February 08, 2020
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Wir beschreiben eine Immunfluoreszenz und quantitative PCR-Methode zur Lokalisierung und Quantifizierung von Begomoviren in Insektengeweben. Das Immunfluoreszenzprotokoll kann zur Kolokalisierung viraler und vektorieller Proteine verwendet werden. Das quantitative PCR-Protokoll kann erweitert werden, um Viren in ganzen Weißfliegenkörpern und virusinfizierten Pflanzen zu quantifizieren.
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Ban, F., Yin, T., Guo, Q., Pan, L., Liu, Y., Wang, X. Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR. J. Vis. Exp. (156), e60731, doi:10.3791/60731 (2020).
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