Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / En forbedret protokol til at rense og direkte…
JoVE Journal
Neuroscience
Author Produced

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
En forbedret protokol til at rense og direkte Mono-Biotinylate Recombinant BDNF i et rør for Cellular Trafficking Studies i Neuroner
Assessment of Thermal Damage from Robot-Drilled Craniotomy for Cranial Window Surgery in Mice
Journal (Neuroscience)
Assessment of Thermal Damage from Robot-Drilled Craniotomy for Cranial Window Surgery in Mice
Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
Journal (Neuroscience)
Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
 
Click here for the English version

En forbedret protokol til at rense og direkte Mono-Biotinylate Recombinant BDNF i et rør for Cellular Trafficking Studies i Neuroner

Article DOI: 10.3791/61262-v • 13:46 min • July 11th, 2020
July 11th, 2020 •
1,2, 1,3, 1,3, 4, 1,3
1Department of Physiology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, 2Department of Molecular and Cellular Biology, Faculty of Biological Sciences, Center for Aging and Regeneration (CARE UC), Pontificia Universidad Católica de Chile, 3Institute of Biomedical Sciences. Faculty of Medicine and Faculty of Life Sciences, Universidad Andrés Bello, 4Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and UK Dementia Research Institute at UCL, University College London Campus

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Rekombinant BDNF indeholdende en Avi-sekvens (BDNFAvi) fremstilles i HEK293-celler på en omkostningseffektiv måde og renses ved affinitetskromatografi. BDNFavi monobiotinyliseres derefter direkte med enzymet BirA i et rør. BDNFavi og mono-biotinyleret BDNFavi bevarer deres biologiske aktivitet sammenlignet med kommercielt tilgængelige BDNF.

Tags

Neurovidenskab BDNF mono-biotinylering quatum prikker axonal handel protein rensning endelsesdynamik in vivo tracking
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter