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Environment

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RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
 

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Article DOI: 10.3791/61477-v 08:55 min May 29th, 2020
May 29th, 2020

Kapitel

Summary

RNA interference is a widely applicable, powerful technique for manipulating gene expression at specific developmental stages. Here, we describe the necessary steps for implementing this technique in the aquatic diving beetle Thermonectus marmoratus, from the acquisition of gene sequences to the knockdown of genes that affect structure or behavior.

Tags

Keywords: RNA Interference Aquatic Beetles Gene Expression Developmental Stages Microinjection Double-stranded RNA Gene Knockdown Non-model Organisms Molecular Genetics
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