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Journal / Immunology and Infection / 囊性纤维化患者痰液中 铜绿假单胞菌…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
囊性纤维化患者痰液中 铜绿假单胞菌 的可视化
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囊性纤维化患者痰液中 铜绿假单胞菌 的可视化

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020
July 16th, 2020 •
1, 2, 1, 1, 1,3, 1,4
1Translational Medicine, Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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Summary
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该方案提供了囊性纤维化患者痰液中细菌细胞和多糖合成位点（Psl）多糖的可视化方法。

Tags

免疫学和感染，第 161 期，慢性感染，肺功能，发病率，抗生素治疗，儿科患者，空间组织，聚集，生物膜形成，多糖产生，痰液样本，组织透明化技术，水凝胶基质，3D 结构，荧光标记，荧光原位杂交，抗 PSL 抗体，胞外多糖，DAPI 染色，宿主细胞，高分辨率成像
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