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Biochemistry

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Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Article DOI: 10.3791/61657-v • 05:59 min • September 26th, 2020
September 26th, 2020 •
1,2, 1,2, 1,2, 1, 1, 1
1Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 2United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University

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Summary
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An ultra-high-speed western blotting technique is developed by improving the kinetics of antigen-antibody binding through cyclic draining and replenishing (CDR) technology in conjunction with an immunoreaction enhancing agent.

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Ultra-High-Speed Western Blot Immunoreaction Enhancing Technology Biomedical Research Block Membrane Sensitivity Antibody Dilution Immunoreaction Enhancing Reagent Incubation Time PVDF Membranes Blocking Buffers Immunoreaction Enhancing Agent Primary Antibody Conical Tube Hybridization Oven
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