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Journal / Neuroscience / Microdissection of Mouse Brain into Functionally…
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Neuroscience

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Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Article DOI: 10.3791/61941-v • 08:06 min • February 15th, 2021
February 15th, 2021 •
1, 1, 2, 3, 3, 2, 4, 2
1The Geneva Foundation, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 2Walter Reed Army Institute of Research, Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, 3ORISE Fellow, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 4Headquarter, Walter Reed Army Institute of Research

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Summary
Automatic Translation

We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs. These brain regions are immediately frozen to obtain high quality transcriptomic data for system level analysis.

Tags

Microdissection Mouse Brain Distinct Brain Regions Molecular Complexity Brain Anatomy Top-down Approach Preservation Of Molecular Integrity Structural Landmarks Training And Practice Mouse Brain Removal Mouse Brain Dissection
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