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Characterization of a Novel Human Organotypic Retinal Culture Technique

Article DOI: 10.3791/62046-v • 05:51 min • June 9th, 2021
June 9th, 2021 •
*1, 1, 1, *1
1Buchanan Ocular Therapeutics Unit, Department of Ophthalmology and the New Zealand National Eye Centre, University of Auckland
* These authors contributed equally

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Summary
Automatic Translation

This study aims to develop a novel human organotypic retinal culture (HORC) model that prevents compromising retinal integrity during explant handling. This is achieved by culturing the retina with the overlying vitreous and the underlying retinal pigment epithelium-choroid (RPE-choroid) and sclera.

Tags

Human Organotypic Retinal Culture Technique Retinal Layers Cell Types Animal Models In Vitro Models Clinical Relevance Retinal Integrity Disruption Healthy And Diseased Retina Retinal Diseases Drug Therapies Dissection Sample Collection Procedures Visual Demonstration Charisse Kuo PhD Student Culture Medium Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Antibiotics And Antimycotics Mixture 24-well Plate Incubator Carbon Dioxide Explant Extraction Eyecup Petri Dish Iris And Lens
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