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A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Article DOI: 10.3791/62127-v • 07:56 min • August 11th, 2021
August 11th, 2021 •
*1, *2, 3, 2
1Department of Medicine, School of Medicine, Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics, University of Florida College of Medicine
* These authors contributed equally

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Summary
Automatic Translation

This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.

Tags

Plate-based Assay Measurement Endogenous Monoamine Release Acute Brain Slices Adaptation Radiolabeled Monoamines Pharmacological Conditions Cost-effective Vesicular Release Transporter Mediated Release Brain Slices Dissection Buffer Coronal Brain Sections Dorsal Striatum Hippocampus Control Slices Experimental Slices Punches Plastic Transfer Pipette
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