Eine kartographische 3D-Beschreibung der Zelle durch Kryo-Soft-Röntgentomographie
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March 15, 2021
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Hier wird ein Protokoll vorgestellt, das die Probenvorbereitungs- und Datenerfassungsschritte beschreibt, die in der Kryo-Soft-Röntgentomographie (SXT) erforderlich sind, um die Ultrastruktur ganzer kryokonservierter Zellen mit einer Auflösung von 25 nm Halbtonhöhe abzubilden.
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Groen, J., Sorrentino, A., Aballe, L., Oliete, R., Valcárcel, R., Okolo, C., Kounatidis, I., Harkiolaki, M., Pérez-Berná, A. J., Pereiro, E. A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography. J. Vis. Exp. (169), e62190, doi:10.3791/62190 (2021).
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