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Immunology and Infection

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Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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Summary
Automatic Translation

We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.

Tags

Purification Expansion Mouse Invariant Natural Killer T Cells In Vitro In Vivo Studies Protocol Rare Cells Ready-to-use Cells Gloria Delfanti Post-doc Mouse Spleen Single Cell Suspension PBS FBS Centrifuge Supernatant Resuspend Sterile Ammonium Chloride Potassium Lysine Buffer Incubate Block Fat Residues Enrichment Steps Cold Cells Pre-cooled Solutions Fc Blocker MACS Separation Buffer
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