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Neuroscience

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JoVE Journal Neuroscience
Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging
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Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging

Article DOI: 10.3791/62267-v • 11:57 min • February 24th, 2021
February 24th, 2021 •
*1, *1, *1, *3, 2, 4,2, 1, 3, *1, *1, *2, *1
1Physics for Medicine Paris, ESPCI Paris, INSERM, CNRS, PSL Research University, 2Institute of Psychiatry and Neurosciences of Paris, INSERM U1266, Université de Paris, 3Iconeus, 4Department of Pharmacology and Toxicology, Jena University Hospital - Friedrich Schiller University Jena
* These authors contributed equally

Kapitel

Summary
Automatic Translation

This protocol describes the quantification of volumetric cerebral hemodynamic variations in the mouse brain using functional ultrasound (fUS). Procedures for 3D functional activation map following sensory stimulation as well as resting-state functional connectivity are provided as illustrative examples, in anesthetized and awake mice.

Tags

Whole-brain 3D Activation Functional Connectivity Mapping Transcranial Functional Ultrasound Imaging Neuroimaging Modality Cerebral Blood Volume Ultra-fast Plane Wave Imaging Hemodynamic Responses Anesthetized Animal Experiments Awake Animal Experiments High Portability Ease Of Use Behavioral Studies Anesthesia Bias Commercially Available Scanners Preclinical Brain Imaging FUS Imaging Eye Ointment Corneal Damage Shave The Mouse Head Depilatory Cream Electrocardiogram Recording Ultrasound Gel
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