Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Analysis of the Lipid Composition of Mycobacteria …
JoVE Journal
Immunology and Infection

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Immunology and Infection
Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Journal (Immunology and Infection)
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Detection of True IgE-expressing Mouse B Lineage Cells
Journal (Immunology and Infection)
Detection of True IgE-expressing Mouse B Lineage Cells
 

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Article DOI: 10.3791/62368-v • 07:42 min • April 16th, 2021
April 16th, 2021 •
1, 1, 1
1Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona

Kapitel

Summary
Automatic Translation

A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.

Tags

Lipid Composition Mycobacteria Thin Layer Chromatography Lipids Extraction Chloroform To Methanol Solution Non-covalent Linked Lipids Immunostimulatory Effect Pathogenicity Host Interactions Mycolic Acid Extraction
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter