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Journal / Immunology and Infection / 流式细胞术分析人髓系U937细胞中SAMHD1限制性…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
流式细胞术分析人髓系U937细胞中SAMHD1限制性
Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
Journal (Immunology and Infection)
Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
Journal (Immunology and Infection)
Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
 
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流式细胞术分析人髓系U937细胞中SAMHD1限制性

Article DOI: 10.3791/62502-v • 09:43 min • June 13th, 2021
June 13th, 2021 •
1, 2, 1, 3
1Retrovirus-Host Interactions Laboratory, The Francis Crick Institute, 2Retroviral Replication Laboratory, The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine, University of Cambridge

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Summary
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这里描述的是一种确定HIV-1限制程度的既定方法，由细胞抑制蛋白SAMHD1。人髓系U937细胞用共表达YFP的SAMHD1表达载体转导，分化，然后用HIV-RFP攻击。限制水平由流式细胞术分析确定。

Tags

免疫学和感染，第172期，人类免疫缺陷病毒-1型（HIV-1），SAMHD1，限制性，骨髓细胞，流式细胞术，逆转录病毒
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