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Journal / Biology / BrdU-DNA標識と細胞周期判別イメージングを用いたグローバルDNA二本鎖末端切除の評価…
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Biology

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BrdU-DNA標識と細胞周期判別イメージングを用いたグローバルDNA二本鎖末端切除の評価
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BrdU-DNA標識と細胞周期判別イメージングを用いたグローバルDNA二本鎖末端切除の評価

Article DOI: 10.3791/62553-v • 06:44 min • April 28th, 2021
April 28th, 2021 •
*1,2, *1,2, 2,3, 1,2
1Oncology Division, Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, 2Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University Cancer Research Center, 3Oncology Division, CHU de Québec Research Center, CHUL Pavilion
* These authors contributed equally

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本プロトコールでは、細胞周期のS/G2期におけるDNA二本鎖末端切除を免疫蛍光ベースの方法を用いて可視化する方法を実証する。

Tags

生物学 BrdU病巣 免疫蛍光 DNA末端切除 相同組換え PARP-1 PARP阻害剤 PARP阻害剤耐性 BMN673
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