Auswahl von Transporter-gezielten inhibitorischen Nanobodies durch Solid-Supported-Membrane (SSM)-basierte Elektrophysiologie
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May 03, 2021
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Nanobodies sind wichtige Werkzeuge in der Strukturbiologie und stellen ein großes Potenzial für die Entwicklung von Therapien dar. Die Auswahl von Nanobodies mit hemmenden Eigenschaften kann jedoch eine Herausforderung darstellen. Hier demonstrieren wir den Einsatz von Solid-Supported-Membrane (SSM)-basierter Elektrophysiologie zur Klassifizierung von inhibitorischen und nicht-inhibitorischen Nanobodies, die auf elektrogene Membrantransporter abzielen.
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Cite this Article
Bärland, N., Perez, C. Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology. J. Vis. Exp. (171), e62578, doi:10.3791/62578 (2021).
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