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The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria

Article DOI: 10.3791/62618-v • 11:05 min • May 3rd, 2021
May 3rd, 2021 •
1
1Department of Physiology, David Geffen School of Medicine, University of California Los Angeles

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Summary
Automatic Translation

This method article details the main steps in measuring H+ leak across the inner mitochondrial membrane with the patch-clamp technique, a new approach to study the thermogenic capacity of mitochondria.

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Patch-clamp Technique Thermogenic Capacity Mitochondria Proton Leak Inner Mitochondrial Membrane Molecular Mechanisms Conductances Calcium Metabolites Adenine Nucleotides Euthanized Mouse Alcohol Spray Incision Thorax Heart Dissection Isolation Solution Isolation Buffer Tissue Homogenization
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