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JoVE Journal
Biochemistry

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Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
 

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Article DOI: 10.3791/62904-v 11:03 min September 9th, 2021
September 9th, 2021

Kapitel

Summary

Fatty acid β-oxidation is an essential metabolic pathway responsible for generating energy in many different cell types, including hepatocytes. Here, we describe a method to measure fatty acid β-oxidation in freshly isolated primary hepatocytes using 14C-labeled palmitic acid.

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Keywords: Fatty Acid Beta-oxidation Mouse Hepatocytes Mitochondrial Peroxisomal Perfusion Collagenase M199 Buffer
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