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Journal / Bioengineering / En högavkastande Streptomyces…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
En högavkastande Streptomyces Transcription-Translation Toolkit för syntetisk biologi och naturliga produktapplikationer
From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow
Journal (Bioengineering)
From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow
Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells
Journal (Bioengineering)
Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells
 
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En högavkastande Streptomyces Transcription-Translation Toolkit för syntetisk biologi och naturliga produktapplikationer

Article DOI: 10.3791/63012-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2,3,4, 5, 5, 1,2,3,4,6,7, 5
1Centre for Synthetic Biology and Innovation, South Kensington Campus, 2Department of Medicine, South Kensington Campus, 3Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, 4Sir Alexander Fleming Building, South Kensington Campus, 5School of Biosciences, Division of Natural Sciences, University of Kent, 6UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, 7UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

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Summary
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Detta protokoll beskriver en förbättrad metod för att syntetisera höga utbyten av rekombinanta proteiner från ett Streptomyces venezuelae cell-free transcription-translation (TX-TL) system.

Tags

Bioengineering nummer 175 Cellfri proteinsyntes in vitro-transkription-översättning Streptomyces syntetisk biologi systembiologi cellfria system biosyntes
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