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Journal / Neuroscience / Digital Droplet PCR Method for the Quantification …
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JoVE Journal Neuroscience
Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina
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Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

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Summary
Automatic Translation

This protocol presents how to quantify AAV transduction efficiency in mouse retina using digital droplet PCR (dd-PCR) together with small scale AAV production, intravitreal injection, retinal imaging, and retinal genomic DNA isolation.

Tags

Digital Droplet PCR AAV Transduction Efficiency Murine Retina Quantification Method Sensitivity Absolute Quantification Target DNA Reporter Gene CRISPR-based Constructs Transduction Rate Straight Forward Results Efficiency Droplet PCR Methodology Quantitative Real-time PCR AAV Titrating Mitochondrial DNA Quantification CRISPR-based Mutation
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