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Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

Article DOI: 10.3791/63049-v • 11:11 min • October 8th, 2021
October 8th, 2021 •
1, 2, 3, 1
1Department of Zoology and Physiology, University of Wyoming, 2School of Electrical Engineering & Computer Science, University of North Dakota, 3Department of Mathematics and Statistics, University of Wyoming

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Summary
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Miniscope in vivo calcium imaging is a powerful technique to study neuronal dynamics and microcircuits in freely behaving mice. This protocol describes performing brain surgeries to achieve good in vivo calcium imaging using a miniscope.

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Stereotaxic Viral Injection Gradient-Index Lens Implantation Deep Brain In Vivo Calcium Imaging Miniscope Spatially Coordinated Activity Temporally Coordinated Activity Freely Behaving Animals Surgery Protocol Viral Injection GRIN Lens Implantation Single Photon Imaging System Two Photon Imaging System Disinfecting Anesthetized Mouse Scalpel Incision Skull Dental Drill Burr Microliter Syringe Air Bubble Virus Flow Rate Needle Brain Tissue
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