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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Article DOI: 10.3791/63072-v • 04:25 min • October 28th, 2021
October 28th, 2021 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2
1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones, Ministry of Education
* These authors contributed equally

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Summary
Automatic Translation

The present protocol describes intratibia osteosarcoma cell injection to generate mouse models bearing orthotopic osteosarcoma and pulmonary metastasis lesions.

Tags

Intratibial Injection Osteosarcoma Cell Mouse Model Orthotopic Osteosarcoma Lung Metastasis Tumor Formation Rates Tumor Volumes Trypsinize Cell Concentration Automatic Cell Counter Centrifuge Cell Pellet Basement Membrane Matrix Injection Site Tibia
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