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Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

Article DOI: 10.3791/63177-v • 10:08 min • December 21st, 2021
December 21st, 2021 •
1, 1, 1
1Laboratory of Genetics, University of Wisconsin-Madison

Kapitel

Summary
Automatic Translation

Early development is dependent on maternally-inherited products, and the role of many of these products is currently unknown. Herein, we described a protocol that uses CRISPR-Cas9 to identify maternal-effect phenotypes in a single generation.

Tags

Maternally-expressed Genes Early Development Maternal CRISPR Technique Maternal Effect Genes Multiplexing Guide RNAs Maternal Effect Phenotypes MRNA Transcripts Gametes Embryogenesis Micro Injection Somatic Mutations Guide RNA Template Oligonucleotide T4 DNA Polymerase DNA Cleanup And Concentrator Kit SgRNA Mixture In-vitro T7 Transcription Kit Agarose Gel Ethidium Bromide Tris-Borate-EDTA Gel
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