Entfernung einer internen translationalen Startstelle aus der mRNA unter Beibehaltung der Expression des Protein in voller Länge
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05:48 min
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March 16, 2022
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Summary
Automatically generated
Das vorliegende Protokoll beschreibt eine einzelne M213L-Mutation in Gja1, die die Connexin43-Generation in voller Länge beibehält, aber die Translation der kleineren GJA1-20k-intern übersetzten Isoform verhindert.
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Shimura, D., Hunter, J., Katsumata, M., Shaw, R. M. Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein. J. Vis. Exp. (181), e63405, doi:10.3791/63405 (2022).
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