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Journal / Bioengineering / In vivo Imágenes de tejidos biológicos con…
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Bioengineering

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JoVE Journal Bioengineering
In vivo Imágenes de tejidos biológicos con fluorescencia combinada de dos fotones y microscopía de dispersión Raman estimulada
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In vivo Imágenes de tejidos biológicos con fluorescencia combinada de dos fotones y microscopía de dispersión Raman estimulada

Article DOI: 10.3791/63411-v • 09:06 min • December 20th, 2021
December 20th, 2021 •
1, 1, 1,2,3,4, 5
1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology

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La microscopía de dispersión Raman estimulada (SRS) permite obtener imágenes sin etiquetas de biomoléculas en función de su vibración intrínseca de enlaces químicos específicos. En este protocolo, se describe la configuración instrumental de un SRS integrado y un microscopio de fluorescencia de dos fotones para visualizar las estructuras celulares en la médula espinal de ratones vivos.

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Bioingeniería Número 178
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