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Journal / Immunology and Infection / Evaluation of a Reliable Biomarker in a Cecal…
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
Journal (Immunology and Infection)
Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Journal (Immunology and Infection)
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
 

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Article DOI: 10.3791/63584-v • 05:28 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1, 1, 1
1Department of Vascular Surgery, Xiangya Hospital, Central South University

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Summary
Automatic Translation

This protocol presents the operative details of cecal ligation and puncture (CLP) in a mouse model of sepsis. CLP is one of the most widely used techniques to create an animal model of sepsis. Therefore, a standardized CLP protocol is required for the attainment of reliable research results.

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Mouse Model Cecal Ligation And Puncture Sepsis Blood Biomarker Citrullinated Histone H3 NETosis Diagnostic Biomarker Male C57BL/6 Mice Anesthesia Depilatory Cream Sterile Surgical Instruments Iodine Disinfection Abdominal Incision Cecum Isolation Silk Suture Ligation Severity Of Sepsis
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