Studying Murine Small Bowel Mechanosensing of Luminal Particulates

Arnaldo Mercado-Perez; Andrew Wegner; Kaitlyn Knutson; Michael Zumchak; Arthur Beyder

doi:10.3791/63697

Journal

/

Medizin

/

Studying Murine Small Bowel Mechanosensing of Luminal Particulates

JoVE Journal
Medizin

Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Medizin

Studying Murine Small Bowel Mechanosensing of Luminal Particulates

DOI:

10.3791/63697-v

•

10:21 min

•

March 18, 2022

•

Arnaldo Mercado-Perez*^1,2, Andrew Wegner*¹, Kaitlyn Knutson, Michael Zumchak, Arthur Beyder⁴

¹Enteric NeuroScience Program (ENSP), Division of Gastroenterology & Hepatology, Department of Medicine,Mayo Clinic, ²Medical Scientist Training Program (MSTP),Mayo Clinic, ³Easy Learning Labs, ⁴Department of Physiology & Biomedical Engineering (PBME),Mayo Clinic

Kapitel

00:05Introduction
00:49Intraluminal Content Gavage and Bowel Dissection
03:42Ex Vivo Imaging and Analysis
07:47Results: Fluorescence Traces Analysis, Geometric Center of Fluorescence Distribution, and Categorization of Power Spectra
09:32Conclusion

Summary

Automatische Übersetzung

To study how the small bowel handles particulates of varying sizes, we have modified an established in vivo method to determine small bowel transit.