Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Measuring Single-Cell Mitochondrial DNA Copy…
JoVE Journal
Medicine

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.

JoVE Journal Medicine
Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
A Murine Model of Subarachnoid Hemorrhage
Journal (Medicine)
A Murine Model of Subarachnoid Hemorrhage
Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
Journal (Medicine)
Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
 

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction

Article DOI: 10.3791/63870-v • 09:15 min • July 12th, 2022
July 12th, 2022 •
1,2, 1,2
1Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus, 2Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus

Kapitel

Summary
Automatic Translation

Here we present a protocol for measuring absolute mitochondrial (mt)DNA copy number and mtDNA deletion heteroplasmy levels in single cells.

Tags

Single-cell Mitochondrial DNA Copy Number Heteroplasmy Digital Droplet PCR Measurement Mutation Tissue Samples Single Cells Mitochondria Isolation Lysis Single Cell Sorting Master Mix PCR Plate Non-template Controls Input DNA Microliters Adhesive Plate Seal Shaker Centrifuge Droplet Generator
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter