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JoVE Journal Genetics
Measuring Caenorhabditis elegans Sensitivity to the Acetylcholine Receptor Agonist Levamisole
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Measuring Caenorhabditis elegans Sensitivity to the Acetylcholine Receptor Agonist Levamisole

Article DOI: 10.3791/64056-v • 08:11 min • June 7th, 2022
June 7th, 2022 •
1, 1
1Department of Biological Sciences, University of Delaware

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Summary
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The present protocol describes an assay to determine response to levamisole, a pharmacological agonist of one class of Caenorhabditis elegans acetylcholine receptors. In this liquid levamisole swim assay, researchers visually observe and quantitate the time-dependent paralysis of animals cultivated in 24-well plates.

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Caenorhabditis Elegans Acetylcholine Receptor Agonist Levamisole Neuromuscular Junction Muscle Function Swimming Behavior Paralysis Worm Counting Nematode Growth Media Sodium Chloride Pep Tone Agar Deionized Water Autoclaving Cholesterol Calcium Chloride Magnesium Sulfate Potassium Phosphate Buffer Well Plate Serological Pipette Bacterial Culture
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