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Endothelial Cell Transcytosis Assay as an In Vitro Model to Evaluate Inner Blood-Retinal Barrier Permeability

Article DOI: 10.3791/64076-v • 10:56 min • June 7th, 2022
June 7th, 2022 •
*1, *1, 1, 1, 1, 1, 1
1Department of Ophthalmology, Boston Children’s Hospital, Harvard Medical School
* These authors contributed equally

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Summary
Automatic Translation

This protocol illustrates an in vitro endothelial cell transcytosis assay as a model to evaluate inner blood-retinal barrier permeability by measuring the ability of human retinal microvascular endothelial cells to transport horseradish peroxidase across cells in caveolae-mediated transcellular transport processes.

Tags

Endothelial Cell Transcytosis Assay In Vitro Model Blood-retinal Barrier Permeability Selective Vascular Barriers Endothelial Transcytosis Molecular Regulators Inner Blood-retinal Barrier Integrity Vascular Biology Eye Research Brain Research Molecule Mechanisms BRB BBB Control Drug Delivery Cell Culture Filter Inserts Human Retinal Microvascular Endothelial Cells HRMECs Gelatin Solution Laminar Flow Hood Petri Dish Growth Media PBS Solution Trypsin-EDTA Solution
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