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In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

DOI:
10.3791/64104-v

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07:03 min

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July 06, 2022

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, , ,
1Department of Cellular Neurobiology, Graduate School of Medicine,The University of Tokyo

Kapitel

  • 00:04Introduction
  • 00:36Implantation of the Observation Window
  • 02:34Two‐Photon Microscopy Immediately After Surgery for Quality Check
  • 03:55In Vivo Chronic Two‐Photon Imaging of Microglia Using CX3CR1‐GFP Mice
  • 04:29Results: In Vivo Imaging of Microglia in CX3CR1‐GFP Mice
  • 06:20Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This paper describes a method for chronic in vivo observation of the resting microglia in the mouse hippocampal CA1 using precisely controlled surgery and two-photon microscopy.

Tags

MicrogliaHippocampusIn VivoTwo-photon ImagingNeuronsCranial WindowExternal CapsuleAlveusCA1Two-photon Microscope

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