Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Development of Knock-Out Muscle Cell Lines using…
JoVE Journal
Genetics

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.

JoVE Journal Genetics
Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
Journal (Genetics)
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells
Journal (Genetics)
Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells
 

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

Kapitel

Summary
Automatic Translation

The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.

Tags

Knock-Out Muscle Cell Lines Lentivirus-Mediated CRISPR/Cas9 Gene Editing Muscle Cell Knock-out Gene Function Cheap And Faster Knock-out Animals Myopathy IPS Cells CRISPR-mediated Gene Deletion Genome Browser Tools Target Gene DNA Sequences Gene Exons Guide RNA Nucleotide Sequences
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter