/
/
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Kapitel
- 00:05Introduction
- 00:59Embryo Collection and Processing
- 03:12Electroporation
- 04:29Embryo Culture and Genotyping
- 05:43Results: Genome Editing by CRISPR RNP Electroporation of Zygotes
- 06:41Conclusion
Here, we describe a simple technique intended for the efficient generation of genetically modified mice called CRISPR RNP Electroporation of Zygotes (CRISPR-EZ). This method delivers editing reagents by electroporation into embryos at an efficiency approaching 100%. This protocol is effective for point mutations, small genomic insertions, and deletions in mammalian embryos.
