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Journal / Biochemistry / Determination of In Vitro and Cellular Turn-on…
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Biochemistry

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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Article DOI: 10.3791/64367-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science, University of Utah
* These authors contributed equally

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Summary
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The protocol presents two methods to determine the kinetics of the fluorogenic RNA aptamers Spinach2 and Broccoli. The first method describes how to measure fluorogenic aptamer kinetics in vitro with a plate reader, while the second method details the measurement of fluorogenic aptamer kinetics in cells by flow cytometry.

Tags

In Vitro Cellular Turn-on Kinetics Fluorogenic RNA Aptamers Quantitative Kinetic Measurements RNA Renaturation Program Thermocycler RNA Solution Renaturation Buffer Plate-reader Injector Program Fluorescence Plate Reader Temperature Equilibration Plate Reader Software
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