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Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel

Article DOI: 10.3791/64563-v • 08:07 min • December 23rd, 2022
December 23rd, 2022 •
1,2, 2,3, 1, 3,5, 6, 2,3,4
1Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center, Maltepe University, 4Department of Histology and Embryology, School of Medicine, Maltepe University, 5Department of Medical Biology, School of Medicine, Maltepe University, 6School of Medicine, Koc University

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Summary
Automatic Translation

The present study describes methodologies for culturing, freezing, thawing, processing, staining, labeling, and examining entire spheroids and organoids under various microscopes, while they remain intact in a hydrogel within a multipurpose device.

Tags

Culturing Freezing Processing Imaging Entire Organoids Spheroids Hydrogel Fragile Accuracy Reproducibility Reliability Repeatability Culture Freeze Thaw Stain Label Examine Microscopes Multi-purpose Device Disease Model Biomarkers Diagnosis Prognosis 3D Growing System Organoids Spheroids Cells Living Organisms
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