Orthotope Implantation von patienteneigenen Krebszellen in Mäusen rekapituliert fortgeschrittenen Darmkrebs

Overview

This protocol describes the orthotopic implantation of patient-derived cancer cells in the cecum wall of immunodeficient mice. This model recapitulates advanced colorectal cancer metastatic disease, allowing for the evaluation of new therapeutic drugs in a clinically relevant scenario of lung and liver metastases.

Key Study Components

Area of Science

• Neuroscience
• Oncology
• Animal Models

Background

• Advanced colorectal cancer is a significant health issue.
• Current models like patient-derived organoids have limitations.
• Orthotopic models provide a more accurate representation of disease.
• This study focuses on a model that mimics clinical scenarios.

Purpose of Study

• To develop a model for studying advanced colorectal cancer.
• To evaluate therapeutic drugs in a relevant biological context.
• To enhance understanding of metastatic processes in colorectal cancer.

Methods Used

• Implantation of patient-derived cancer cells in mice.
• Use of immunodeficient mice to support tumor growth.
• Extraction and dissociation of tumors for analysis.
• Maintenance of tumor samples in PBS for further experimentation.

Main Results

• The model successfully recapitulates advanced metastatic disease.
• Therapeutic evaluations can be conducted in this model.
• Insights into tumor biology and metastasis are gained.
• The model demonstrates potential for future research applications.

Conclusions

• The orthotopic model is a valuable tool for colorectal cancer research.
• It allows for the study of advanced disease and therapeutic responses.
• This approach may lead to improved treatment strategies.

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