Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Quantifying Myeloperoxidase-DNA and Neutrophil…
JoVE Journal
Immunology and Infection

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.

JoVE Journal Immunology and Infection
Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
Journal (Immunology and Infection)
Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
Journal (Immunology and Infection)
Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
 

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Article DOI: 10.3791/64644-v • 07:18 min • May 12th, 2023
May 12th, 2023 •
1, 2, 2, 2, 2
1Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), 2Department of Emergency and Critical Care Medicine, Aichi Medical University

Kapitel

Summary
Automatic Translation

We present a protocol for a modified sandwich enzyme-linked immunosorbent assay technique to quantitatively measure two components of neutrophil extracellular trap remnants, myeloperoxidase conjugated-DNA and neutrophil elastase conjugated-DNA complexes,derived from activated neutrophils.

Tags

Myeloperoxidase-DNA Neutrophil Elastase-DNA Neutrophil Extracellular Traps (NETs) Sandwich ELISA Clinical Settings Sepsis Septic ARDS Acute Respiratory Distress Syndrome Dumps Or Damage-associated Molecular Patterns Circulating NET Remnants Incubation Times Polymorphonuclear Neutrophils (PMNs) Phenol Red-free RPMI 1640 PMA Stimulation DNase I Digestion EDTA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter