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Modelado del carcinoma de células escamosas oral-esofágico en organoides 3D
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1Herbert Irving Comprehensive Cancer Center,Columbia University, 2Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center,Columbia University, 3Department of Otolaryngology, Head and Neck Surgery,Columbia University, 4Histopathology Facility,Fox Chase Cancer Center, 5Division of Digestive and Liver Diseases, Department of Medicine,Columbia University, 6Department of Genetics and Development,Columbia University, 7Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine,Columbia University
Kapitel
- 00:04Introduction
- 01:28Animal Dissection and Organ Collection
- 04:39Isolation of Keratinocytes from the Dissected Mouse Tissue
- 06:07Seeding of the Initial Cell Suspension
- 06:54Passaging and Cryopreservation of Murine Esophageal Organoids (MEOs)
- 07:52Preparation of Organoids for Paraffin Embedding
- 08:48Results: Characterization of the Organoids
- 10:03Conclusion
