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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Article DOI: 10.3791/64825-v • 04:04 min • January 20th, 2023
January 20th, 2023 •
*1, *1,2, 1,2,3
1Department of Physics and Astronomy, University of California, 2Microbiology Program, University of California, 3Biophysics Program, University of California
* These authors contributed equally

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Summary
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A protocol is outlined to perform live real-time imaging to quantify how the accessory protein TnpB affects the dynamics of transposition in individual live Escherichia coli cells.

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Real-Time Quantification Effects IS200/IS605 Family-Associated TnpB Transposon Activity Quantify Excision Statistics Single-cell Basis Bulk Behavior Track Behavior Individual Cells TnpA Levels TnpB Levels Excise Measure Effects Identified Mutational Events Growth Rate Simple Method Low-cost Way Live Cell Mutational Dynamics Live Cell Fluorescent Imaging Prepare Slide Boiling M63 Glucose Agarose Microwave Melt Agarose Cool To 55 Degrees Celsius Antibiotics Inducers Microscope Slide Gap Between Slides Gel Square Solidify Gel Remove Top Slide Trim Agarose Pad Razor Blade Or Knife Bacterial Culture Cover Slip
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