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Estabelecimento e Cultura de Organoides Mamários Derivados da Paciente
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Estabelecimento e Cultura de Organoides Mamários Derivados da Paciente

Article DOI: 10.3791/64889-v • 08:29 min • February 17th, 2023
February 17th, 2023 •
1,2, 1, 1, 1, 1,2
1Cold Spring Harbor Laboratory, Cold Spring Harbor, 2Genetics Graduate Program, Stony Brook University

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Um protocolo detalhado é fornecido aqui para estabelecer organoides mamários humanos a partir de ressecções de tumor de mama derivadas de pacientes ou tecido mamário normal. O protocolo fornece instruções passo a passo abrangentes para cultivar, congelar e descongelar organoides mamários derivados de pacientes humanos.

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