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Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
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Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Article DOI: 10.3791/65040-v • 10:49 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 1,2, 1, 1, 1,2
1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), University Medical Center Utrecht, 2Oncode Institute, Hubrecht Institute

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Summary
Automatic Translation

This protocol describes how to establish, maintain, genetically modify, differentiate, functionally characterize, and transplant lacrimal gland organoids derived from primary mouse and human tissue.

Tags

Lacrimal Gland Organoids Genes Homeostasis Regenerative Medicine Stem Cells Epithelial Cells Dry Eye Disease Medium Preparation Material Preparation Digestion Medium Tissue Mincing 15-milliliter Tube Water Bath Dissociation Process
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