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Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Article DOI: 10.3791/65053-v • 09:26 min • June 30th, 2023
June 30th, 2023 •
1,2,3, 4,5,6, 3, 3, 1,2, 1,2, 1,2, 2,3, 4,5, 1,2
1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)

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Summary
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This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.

Tags

Quantification Whole Genome Characterization SARS-CoV-2 RNA Wastewater-based Epidemiology Viral RNA COVID-19 Control Strategies Potential Outbreaks Population-wide Snapshot Asymptomatic Cases Air Sampling Clinical Diagnostics Disease Outbreaks Pandemics Molecular Biology Mengovirus Centrifugation Ultrafiltration Device RNA Extraction Air Samples
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