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Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

Article DOI: 10.3791/65104-v • 06:00 min • February 3rd, 2023
February 3rd, 2023 •
*1,4, *2, 2, 3, 4, 4, 1, 1
1Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV, 2Cell and Tissue Engineering Lab, IRCCS Ospedale Galeazzi Sant'Ambrogio, 3Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine, Lausanne University Hospital, 4Centre for the Cellular Microenvironment, University of Glasgow
* These authors contributed equally

Kapitel

Summary
Automatic Translation

Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described.

Tags

Isolation Culture Human Adipose-derived Stem Cells Xenogeneic-free Method Biosafety Concerns Cell Therapy Translational Application In Vitro Expansion Peripheral Nerve Repair Clinical Research Setups Producibility Sterility Sample Manipulation Fragments Micro Dissection Adipose Tissue Sample Petri Dish Complete Culture Medium Incubate
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