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Journal
/
Biologie
/
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
/
Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
DOI:
10.3791/65219-v
•
02:35 min
•
April 07, 2023
•
Yuta Takase
,
Kazuto Fujishima
3
,
Toshio Takahashi
1
Suntory Foundation for Life Sciences
,
Bioorganic Research Institute
,
2
Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS)
,
Kyoto University
,
3
Faculty of Medicine
,
Osaka Medical and Pharmaceutical University
Tags
3D Organoids
Intestinal Crypts
Intestinal Stem Cells
Homeostasis
Epithelial Growth
Differentiation
EDTA
Mechanical Dissociation
Signaling
Intestinal Epithelium
Paneth Cells
Enteroendocrine Cells
Enterocytes
Goblet Cells
Tuft Cells
Leucine-rich Repeats Containing G Protein-coupled Receptor 5
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Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
Processing Murine Small Intestine for Crypt Isolation
Crypt Isolation from Murine Small Intestine
Organoid Culture from a Single Intestinal Stem Cell
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