Verwendung von Lebendzell-STED-Bildgebung zur Visualisierung der mitochondrialen Innenmembran-Ultrastruktur in neuronalen Zellmodellen
3,663 Views
•
08:48 min
•
June 30, 2023
DOI:
Summary
Automatically generated
Dieses Protokoll stellt einen Arbeitsablauf für die Vermehrung, Differenzierung und Färbung von kultivierten SH-SY5Y-Zellen und primären Hippocampusneuronen der Ratte für die Visualisierung und Analyse der mitochondrialen Ultrastruktur unter Verwendung der stimulierten Emissionsverarmungsmikroskopie (STED) dar.
Explore More Videos
Verwandte Videos
04:50
Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
Verwandte Videos
16756 Views
08:46
A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
Verwandte Videos
1581 Views
07:47
Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
Verwandte Videos
14024 Views
07:32
Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
Verwandte Videos
7755 Views
N/A
Live Animal Imaging and Cell Sorting Methods for Investigating Neurodegeneration in a C. elegans Excitotoxic Necrosis Model
Verwandte Videos
1825 Views
06:20
Live-imaging of Mitochondrial System in Cultured Astrocytes
Verwandte Videos
3946 Views
07:47
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Verwandte Videos
2795 Views
05:29
Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging
Verwandte Videos
800 Views
05:53
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
Verwandte Videos
11.2K Views
08:29
Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
Verwandte Videos
11.3K Views
Read Article
Cite this Article
Ng, E. L., Reed, A. L., O’Connell, C. B., Alder, N. N. Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models. J. Vis. Exp. (196), e65561, doi:10.3791/65561 (2023).
Copy