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JoVE Journal Neuroscience
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Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons (Video) | JoVE

Culturing Human Midbrain Dopaminergic Neurons for Phenotypic Profiling

Article DOI: 10.3791/65570-v • 02:16 min • July 7th, 2023
July 7th, 2023 •
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Phenotypic Profiling Human Stem Cells Midbrain Dopaminergic Neurons Parkinson's Disease In Vitro PD Cellular Models Induced Pluripotent Stem Cells (iPSC) Neuronal Phenotypes Fluorescent Staining Panel Nuclear Staining α-synuclein Tyrosine Hydroxylase (TH) Microtubule-associated Protein 2 (MAP2) Phenotypic Profiling Protocol Scalable 384-well Plates Automatic Liquid Handling High-throughput Microscopy G2019S Mutation Leucine-rich Repeat Kinase 2 (LRRK2) Gene LRRK2 Kinase Inhibitor PFE-360 Phenotypic Changes Multidimensional Phenotypic Profiles Clustering Analysis Machine Learning-driven Supervised Classification
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