Measuring Mitochondrial Function of Na&#239;ve and Effector CD8 T Cells

Jacqueline  A. Turner; Elizabeth Katsnelson; Raul M. Torres

doi:10.3791/65642

Messung der mitochondrialen Funktion von naiven und Effektor-CD8-T-Zellen

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Messung der mitochondrialen Funktion von naiven und Effektor-CD8-T-Zellen

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06:07 min

March 28, 2025

DOI: 10.3791/65642-v

Jacqueline A. Turner^1,2, Elizabeth Katsnelson³, Raul M. Torres¹

¹Department of Immunology and Microbiology,University of Colorado School of Medicine, Anschutz Medical Campus, ²Medical Scientist Training Program,University of Colorado School of Medicine, Anschutz Medical Campus, ³Division of Surgical Oncology, Department of Surgery,University of Colorado School of Medicine, Anschutz Medical Campus

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Overview

This study investigates the impact of lysophosphatidic acid on CD8 T cell cytotoxicity and metabolic flexibility. The methodology includes the Mito Stress Test to analyze T cell bioenergetics and receptor signaling.

Key Study Components

Area of Science

Immunology
Cell Metabolism
T cell Biology

Background

CD8 T cells play a crucial role in immune responses.
Lysophosphatidic acid affects T cell function and metabolism.
Understanding metabolic programming is essential for immune tolerance.
Bioenergetic analysis can reveal insights into T cell behavior.

Purpose of Study

To examine the effects of lysophosphatidic acid on CD8 T cell metabolism.
To assess the relationship between T cell receptor signaling and bioenergetics.
To utilize real-time analysis for functional metabolism assessment.

Methods Used

Mito Stress Test for bioenergetic profiling.
Measurement of mitochondrial metabolism in CD8 T cells.
Experimental protocols for assessing metabolic flexibility.
Real-time analysis of T cell receptor signaling.

Main Results

Lysophosphatidic acid reduces metabolic flexibility in CD8 T cells.
Altered metabolic fitness influences T cell phenotypes.
Real-time assessments provide insights into T cell bioenergetics.
Findings suggest potential therapeutic targets for immune modulation.

Conclusions

Lysophosphatidic acid is a key regulator of CD8 T cell metabolism.
Understanding metabolic changes can inform immune tolerance strategies.
Future studies may explore therapeutic implications for T cell modulation.

Frequently Asked Questions

What is the Mito Stress Test?

The Mito Stress Test is a methodology used to assess mitochondrial function and bioenergetics in cells.

How does lysophosphatidic acid affect T cells?

Lysophosphatidic acid disrupts CD8 T cell cytotoxicity and reduces metabolic flexibility.

What is the significance of metabolic flexibility in T cells?

Metabolic flexibility allows T cells to adapt their energy production based on environmental cues, which is crucial for effective immune responses.

Why is real-time analysis important in this study?

Real-time analysis allows for immediate observation of metabolic changes and T cell receptor signaling dynamics.

What are the potential therapeutic implications of this research?

Understanding the metabolic regulation of T cells may lead to new strategies for enhancing immune responses or promoting tolerance.

Die Bioenergetik der CD8-T-Zellen kann mit dem Mito Stresstest abgefragt werden. Diese Methodik kann verwendet werden, um die akute und chronische metabolische Programmierung zu untersuchen. Dieses Protokoll beschreibt Ansätze zur Untersuchung der Beziehungen zwischen der Biologie von T-Zell-Rezeptoren und der bioenergetischen Analyse.

Unser Labor untersucht die Immuntoleranz, die Immunüberwachung und die Antigenrezeptor-Signalgebung durch Lymphozyten. Wir fanden heraus, dass Lysophosphatidsäure die Zytotoxizität von CD8-T-Zellen stört und die periphere Toleranz fördert, indem sie die metabolische Flexibilität in Effektorzellen reduziert. Wir verwenden Technologien, um den mitochondrialen Stoffwechsel und die Energiekapazität in CD8-T-Zellen zu messen.

Unser Labor hat gezeigt, dass Lysophosphatidsäure die metabolische Fitness von CD8-T-Zellen moduliert und eine wichtige Determinante für T-Zell-Phänotypen sein kann. Unser hier beschriebenes Protokoll bewertet den funktionellen Stoffwechsel und die Bioenergetik der T-Zell-Rezeptor-Signalübertragung in Echtzeit. Geben Sie zunächst 200 Mikroliter destilliertes Wasser in jede Vertiefung einer Versorgungsplatte.

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